Protein Name & Synonyms:
BCAM, AU, CD239, LU, MSK19, basal cell adhesion molecule
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
This gene encodes Lutheran blood group glycoprotein, a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five extracellular immunoglobulin domains, a single transmembrane domain, and a short C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Polymorphisms in this gene define some of the antigens in the Lutheran system and also the Auberger system. Inactivating variants of this gene result in the recessive Lutheran null phenotype, Lu(a-b-), of the Lutheran blood group. Two transcript variants encoding different isoforms have been found for this gene.
WB 1:500 - 1:2000
Recombinant fusion protein containing a sequence corresponding to amino acids 352-547 of human BCAM (NP_005572.2).
Western blot analysis of extracts of mouse lung, using BCAM antibody (144-60773) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 60s.