ELISA (recommended work dilution= 1: 32,000), Western Blotting (recommended work dilution= 1: 1,000)
The immunogen was a synthetic peptide (BIScV-Coat protein). This antibody was produced from a RABBIT immunized with this peptide conjugated with KLH. The IgG fraction was purified from rabbit serum by Protein G affinity chromatography.
A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial.
Blueberry scorch virus (BlScV) was first found in B.C. in 2000, but it is likely that BlScV was present in B.C. several years earlier. It is now widespread in all blueberry growing areas of the province. Severity of the symptoms depends on the cultivar and viral strain, but all highbush blueberry varieties grown in B.C. appear to be susceptible. BlScV can cause severe blossom and leaf blighting, and decreased yields. Once infected, plants do not recover. Infected plants show symptoms each year, and should be removed. BlScV is spread by aphids or planting infected stock.
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
This antibody specifically binds to BIScV-Coat protein.