ELISA (recommended work dilution= 1: 32,000), Western Blotting (recommended work dilution= 1: 1,000)
The immunogen was a synthetic peptide (BIScV-Coat protein). This antibody was produced from a RABBIT immunized with this peptide conjugated with KLH. The IgG fraction was purified from rabbit serum by Protein G affinity chromatography.
Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile 1 x PBS to a final concentration of 1 mg/ml.
Blueberry scorch virus (BlScV) was first found in B.C. in 2000, but it is likely that BlScV was present in B.C. several years earlier. It is now widespread in all blueberry growing areas of the province. Severity of the symptoms depends on the cultivar and viral strain, but all highbush blueberry varieties grown in B.C. appear to be susceptible. BlScV can cause severe blossom and leaf blighting, and decreased yields. Once infected, plants do not recover. Infected plants show symptoms each year, and should be removed. BlScV is spread by aphids or planting infected stock.
This antibody specifically binds to BIScV-Coat protein.
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can also be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for at least 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 30 days at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.