Protein Name & Synonyms:
BMI1, FLVI2/BMI1, PCGF4, RNF51, flvi-2/bmi-1, polycomb complex protein BMI-1
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
This gene encodes a ring finger protein that is major component of the polycomb group complex 1 (PRC1). This complex functions through chromatin remodeling as an essential epigenetic repressor of multiple regulatory genes involved in embryonic development and self-renewal in somatic stem cells. This protein also plays a central role in DNA damage repair. This gene is an oncogene and aberrant expression is associated with numerous cancers and is associated with resistance to certain chemotherapies. A pseudogene of this gene is found on chromosome X. Read-through transcription also exists between this gene and the upstream COMM domain containing 3 (COMMD3) gene.
WB 1:500 - 1:2000
IP 1:50 - 1:100
Recombinant fusion protein containing a sequence corresponding to amino acids 120-326 of human BMI1 (NP_005171.4).
Western blot analysis of extracts of various cell lines, using BMI1 antibody (144-00211) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Immunoprecipitation analysis of 200ug extracts of K-562 cells, using 3 ug BMI1 antibody (144-00211). Western blot was performed from the immunoprecipitate using BMI1 antibody (144-00211) at a dilition of 1:1000.