Rabbit Anti-Bovine Smooth Muscle Myosin II

Rabbit Anti-Bovine Smooth Muscle Myosin II

$571.00
DS-PB-01823
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Antigen Information

Protein Name & Synonyms:
Smooth Muscle Myosin II
Target Species:
  • Bovine (cow)

Antibody Specifications

Applications:
Immunohistology - Frozen, Immunofluorescence, Western Blotting
Preparation:
Antisera to bovine smooth muscle myosin II were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.
Species Reactivity:
Bovine, Mouse

Specificity

This product specifically recognizes the heavy chain of myosin II found in vertebrate smooth muscle, a non-striated muscle present in the walls of hollow organs, the vasculature, the respiratory and gastrointestinal tracts, uterus, bladder and eye.

The structure of myosin II is fundamentally the same in all muscle types and consists of two heavy chains, each bound by 2 distinct, essential and regulatory light chains. The globular motor domain located at the N-terminal domain of the heavy chains, catalyzes ATP hydrolysis and interacts with actin, whilst the heptad repeat sequences of the heavy chain tail domains promote dimerization, forming an alpha-helical coiled coil at the C-terminal domain.

This antibody does not cross-react with myosin of skeletal or non-muscle origin and can be used to specifically stain cultured smooth muscle cells and identify vascular and other smooth muscle types in tissue sections.

Storage/Stability

Store at -20 °C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
TRIS buffered saline
Format Type:
Purified

Preparation

Antisera to bovine smooth muscle myosin II were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by ion exchange chromatography.

Immunogen:
Bovine tracheal smooth muscle myosin.
Preservative:
0.006%Sodium Azide (NaN3)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.