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Antigen Information
Protein Name & Synonyms:
BRCC36
Target Species:
- Human
Antibody Specifications
Applications:
Immunohistology - Paraffin, Western Blotting
Concentration:
IgG concentration 1.0mg/ml
Preparation:
Antiserum to human BRCC36 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Species Reactivity:
Human, Mouse, Rat
This product detects a band of approximately 36kDa in MCF7 cell lysates.
Specificity
This antibody recognizes human BRCA1/BRCA2 complex subunit 36 (BRCC36), a 36.7kDa nuclear protein.
BRCC36, together with BRCA1, BRCA2, Rad51 and BRCC45, forms a holoenzyme complex that possesses an ubiquitin E3 ligase activity.
Studies have demonstrated that BRCC36 functions in enhancing cellular survival following DNA damage and that it is essential for ionizing radiation induced BRCA1 phosphorylation and nuclear foci formation.
Research has shown that BRCC36 is aberrantly expressed in the majority of breast tumours indicating a potential role in the pathogenesis of the disease. It has been hypothesized that BRCC36 could be a potentially important target in the treatment of radiation-resistant breast tumours.
BRCC36, together with BRCA1, BRCA2, Rad51 and BRCC45, forms a holoenzyme complex that possesses an ubiquitin E3 ligase activity.
Studies have demonstrated that BRCC36 functions in enhancing cellular survival following DNA damage and that it is essential for ionizing radiation induced BRCA1 phosphorylation and nuclear foci formation.
Research has shown that BRCC36 is aberrantly expressed in the majority of breast tumours indicating a potential role in the pathogenesis of the disease. It has been hypothesized that BRCC36 could be a potentially important target in the treatment of radiation-resistant breast tumours.
Storage/Stability
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Format
Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified
Preparation
Antiserum to human BRCC36 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Immunogen:
An 18 amino acid peptide from near the amino terminus of human BRCC36.
Preservative:
0.02% Sodium Azide (NaN3)
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
