Protein Name & Synonyms:
CAPN1, CANP, CANP1, CANPL1, SPG76, muCANP, muCL, calpain 1
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 1. Several transcript variants encoding two different isoforms have been found for this gene.
WB 1:500 - 1:2000
Recombinant fusion protein containing a sequence corresponding to amino acids 20-330 of human CAPN1 (NP_005177.2).
Western blot analysis of extracts of various cell lines, using CAPN1 antibody (144-60042) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
12 months from the date of shipment when stored properly.