Protein Name & Synonyms:
CCNK, CPR4, cyclin-K
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Human, Mouse, Rat
The protein encoded by this gene is a member of the transcription cyclin family. These cyclins may regulate transcription through their association with and activation of cyclin-dependent kinases (CDK) that phosphorylate the C-terminal domain (CTD) of the large subunit of RNA polymerase II. This gene product may play a dual role in regulating CDK and RNA polymerase II activities.
WB 1:500 - 1:2000
Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human CCNK (NP_001092872.1).
Western blot analysis of extracts of various cell lines, using CCNK Antibody (144-010261) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.