The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene.
WB 1:500 - 1:2000
IF 1:50 - 1:200
Recombinant protein of human CD34
Western blot analysis of extracts of various cell lines, using CD34 antibody (144-07429) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunofluorescence analysis of HUVEC cells using CD34 antibody (144-07429) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
12 months from the date of shipment when stored properly.