Rabbit anti-CFD Antibody

CFD Polyclonal Antibody

144-08117-50
+

Antigen Information

Accession Number:
  • P00746
Gene ID:
  • 1675
Gene Symbols:
  • CFD
Protein Name & Synonyms:
CFD, ADIPSIN, ADN, DF, PFD, complement factor D
Target Species:
  • Human

Antibody Specifications

Applications:
WB,IHC
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugation/Tag:
Unconjugated
Host Species:
Rabbit
Purity:
Affinity purification
Species Reactivity:
Human,Mouse
Clonality:
Polyclonal Antibodies
Isotype:
IgG

Background

This gene encodes a member of the S1, or chymotrypsin, family of serine peptidases. This protease catalyzes the cleavage of factor B, the rate-limiting step of the alternative pathway of complement activation. This protein also functions as an adipokine, a cell signaling protein secreted by adipocytes, which regulates insulin secretion in mice. Mutations in this gene underlie complement factor D deficiency, which is associated with recurrent bacterial meningitis infections in human patients. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature protease.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW
27kDa
Observed MW
27kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 26-253 of human CFD (NP_001919.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using CFD antibody (144-08117) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 20s.
Western blot - CFD antibody (144-08117)

Immunohistochemistry of paraffin-embedded human prostate using CFD antibody (144-08117) at dilution of 1:100 (40x lens).
Immunohistochemistry - CFD antibody (144-08117)

Immunohistochemistry of paraffin-embedded human stomach using CFD antibody (144-08117) at dilution of 1:100 (40x lens).
Immunohistochemistry - CFD antibody (144-08117)

No posts found

Write A Review
U.S. Non-U.S.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.