Protein Name & Synonyms:
CFD, ADIPSIN, ADN, DF, PFD, complement factor D
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
This gene encodes a member of the S1, or chymotrypsin, family of serine peptidases. This protease catalyzes the cleavage of factor B, the rate-limiting step of the alternative pathway of complement activation. This protein also functions as an adipokine, a cell signaling protein secreted by adipocytes, which regulates insulin secretion in mice. Mutations in this gene underlie complement factor D deficiency, which is associated with recurrent bacterial meningitis infections in human patients. Alternative splicing of this gene results in multiple transcript variants. At least one of these variants encodes a preproprotein that is proteolytically processed to generate the mature protease.
WB 1:500 - 1:2000
IHC 1:50 - 1:100
Recombinant fusion protein containing a sequence corresponding to amino acids 26-253 of human CFD (NP_001919.2).
Western blot analysis of extracts of various cell lines, using CFD antibody (144-08117) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 20s.
Immunohistochemistry of paraffin-embedded human prostate using CFD antibody (144-08117) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using CFD antibody (144-08117) at dilution of 1:100 (40x lens).