Protein Name & Synonyms:
CFHR3, CFHL3, DOWN16, FHR-3, FHR3, HLF4, complement factor H related 3
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
The protein encoded by this gene is a secreted protein, which belongs to the complement factor H-related protein family. It binds to heparin, and may be involved in complement regulation. Mutations in this gene are associated with decreased risk of age-related macular degeneration, and with an increased risk of atypical hemolytic-uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
WB 1:500 - 1:2000
Recombinant fusion protein containing a sequence corresponding to amino acids 80-200 of human CFHR3 (NP_066303.2).
Western blot analysis of extracts of various cell lines, using CFHR3 antibody (144-07775) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.