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770-729-2992
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Product Specifications
Size:
0.5 mg
Protein Information
Protein Name & Synonyms:
IFN-alpha
Target Species:
- Chicken
Antibody Specifications
Applications:
ELISA, Functional Assays, Immunoprecipitation, Western Blotting
Concentration (lot specific):
IgG concentration 0.5 mg/ml
Preparation:
Antisera to chicken Interferon alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography on protein G.
Species Reactivity:
Chicken
This product detects a band of approximately 19kDa.
Specificity
This product recognizes chicken Interferon alpha (chIFN-alpha).
The classification of mammalian cytokines with antiviral activity, is well documented as being either Type I Interferon (IFNI) or Type II Interferon (IFNII). This is not the case for avian IFNs however, even though IFN was first detected in the chicken.
A growing number of functional studies indicate similarities between chicken IFN and mammalian IFNI, revealing the conservation of cysteine residues and the existence of around ten closely related intron-less IFN alpha genes. Further research has shown that chIFN-alpha genes are strongly expressed following viral infection of monocyte-derived macrophage and embryo fibroblasts and that recombinant chicken IFN acts as a potent antiviral agent.
This product cross reacts with IFN beta in western blots but does not show any cross reactivity with IFN gamma. In bioassays, this antiserum neutralizes chIFN-alpha, but not chIFN-beta, and can therefore be used to discriminate IFN alpha and IFN beta bioactivities.
The classification of mammalian cytokines with antiviral activity, is well documented as being either Type I Interferon (IFNI) or Type II Interferon (IFNII). This is not the case for avian IFNs however, even though IFN was first detected in the chicken.
A growing number of functional studies indicate similarities between chicken IFN and mammalian IFNI, revealing the conservation of cysteine residues and the existence of around ten closely related intron-less IFN alpha genes. Further research has shown that chIFN-alpha genes are strongly expressed following viral infection of monocyte-derived macrophage and embryo fibroblasts and that recombinant chicken IFN acts as a potent antiviral agent.
This product cross reacts with IFN beta in western blots but does not show any cross reactivity with IFN gamma. In bioassays, this antiserum neutralizes chIFN-alpha, but not chIFN-beta, and can therefore be used to discriminate IFN alpha and IFN beta bioactivities.
Storage/Stability
Store at -20 °C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Format
Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Azide Free
Preparation
Antisera to chicken Interferon alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography on protein G.
Immunogen:
Recombinant chicken Interferon alpha.
Preservative:
None present
Expiration:
12 months from the date of shipment when stored properly.
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
