Rabbit Anti-E. coli [+FITC]

Rabbit Anti-E. coli [+FITC]

DS-PB-00729
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Antigen Information

Protein Name & Synonyms:
E. coli
Target Species:
  • Bacterial

Antibody Specifications

Applications:
Immunofluorescence
Concentration:
IgG concentration 4.0 mg/ml
Species Reactivity:
Bacterial

Specificity

This product recognizes Escherichia coli and is broadly reactive with all somatic and capsular ("O" and "K") antigenic serotypes. The somatic "O" antigens are composed of lipopolysaccharide complexes which form part of the cell wall structure of E. coli whilst the capsular "K" antigens are mainly composed of acidic polysaccharide.

The antibody will remove E.coli proteins from recombinant preparations. It is unabsorbed and cross reacts with related enterobacteriaceae.

The unconjugated antibody has been used in ELISA to detect serotypes O157:H7, O20, O125, O55, O111 and K12.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Formulation:
Phosphate buffered saline

Preparation

Immunogen:
Raised against a mixture of all antigenic serotypes
Preservative:
0.1%Sodium Azide (NaN3)
1%Bovine Serum Albumin

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.