Rabbit anti-EHHADH Antibody

EHHADH Polyclonal Antibody
CODE:  144-05717-50

Antigen Information

Accession Number:
  • Q08426
Gene ID:
  • 1962
Gene Symbols:
  • EHHADH
Protein Name & Synonyms:
EHHADH, ECHD, FRTS3, L-PBE, LBFP, LBP, PBFE, peroxisomal bifunctional enzyme
Target Species:
  • Human

Antibody Specifications

Applications:
WB
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugation/Tag:
Unconjugated
Host Species:
Rabbit
Purity:
Affinity purification
Species Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal Antibodies
Isotype:
IgG

Background

The protein encoded by this gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for this gene.

Dilutions

WB 1:500 - 1:2000

Format

Calculated MW
69kDa/79kDa
Observed MW
79kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 444-723 of human EHHADH (NP_001957.2).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using EHHADH antibody (144-05717) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
Western blot - EHHADH antibody (144-05717)