Protein Name & Synonyms:
TNF-alpha / TNF
Applications:
ELISA (dilution: 0.1ug/ml, 1.0ug/ml), Western Blotting
Concentration:
IgG concentration 1.0 mg/ml
Preparation:
Antisera to equine TNF alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography on protein G.
Species Reactivity:
Horse
This product is specific for equine tumour necrosis factor alpha (TNF alpha).
TNF alpha is produced by a range of cells, including macrophages and monocytes and is an important mediator of the equine inflammatory response.
This product recognizes both native and recombinant equine TNF alpha. No detectable cross-reactivity was observed with bovine, porcine, mouse or rat TNF alpha.
Store at +4 °C or -20 °C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Supplied as:
Purified IgG conjugated to Biotin - liquid
Formulation:
Phosphate buffered saline
Antisera to equine TNF alpha were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography on protein G.
Immunogen:
Recombinant equine TNF alpha.