Protein Name & Synonyms:
ERLIN1, C10orf69, Erlin-1, KE04, KEO4, SPFH1, SPG62, erlin-1
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
The protein encoded by this gene is part of a protein complex that mediates degradation of inositol 1,4,5-trisphosphate receptors in the endoplasmic reticulum. The encoded protein also binds cholesterol and regulates the SREBP signaling pathway, which promotes cellular cholesterol homeostasis. Defects in this gene have been associated with spastic paraplegia 62.
WB 1:500 - 1:2000
Recombinant fusion protein containing a sequence corresponding to amino acids 259-348 of human ERLIN1 (NP_006450.2).
Western blot analysis of extracts of various cell lines, using ERLIN1 antibody (144-60819) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.