Protein Name & Synonyms:
EXOSC1, CGI-108, CSL4, Csl4p, SKI4, Ski4p, p13, exosome component 1
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
This gene encodes a core component of the exosome. The mammalian exosome is required for rapid degradation of AU rich element-containing RNAs but not for poly(A) shortening. The association of this protein with the exosome is mediated by protein-protein interactions with ribosomal RNA-processing protein 42 and ribosomal RNA-processing protein 46. Alternative splicing of this gene results in multiple transcript variants.
WB 1:500 - 1:2000
Recombinant fusion protein containing a sequence corresponding to amino acids 1-195 of human EXOSC1 (NP_057130.1).
Western blot analysis of extracts of various cell lines, using EXOSC1 antibody (144-10302) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.