Western blot analysis of extracts of various cell lines, using FKBP8 antibody (144-07085) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.
Immunofluorescence analysis of A549 cells using FKBP8 antibody (144-07085). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug FKBP8 antibody (144-07085). Western blot was performed from the immunoprecipitate using FKBP8 antibody (144-07085) at a dilition of 1:1000.
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