Protein Name & Synonyms:
Western blotting (0.5-4 ug/ml), immunoprecipitation (10-20 ug/ml), and Immunohistochemistry (10-20 ug/ml). However, the optimal concentrations should be determined individually. The antibody recognizes 25 kDa GADD153 of human, mouse, and rat origins.
GADD153 , DDIT3 , 126337 ,P35638 , CEBPZ , CHOP , CHOP10 , GADD153 , MGC4154
GADD153 has been described as a growth arrest and DNA damage-inducible gene that encodes a C/EBP-related nuclear protein. Expression of GADD153 is induced by a variety of cellular stresses, including nutrient deprivation and metabolic perturbations. GADD153 functions to block cells in G1 to S phase in cell cycle progression and acts by dimerizing with other C/EBP proteins to direct GADD153 dimers away from classical C/EBP binding sites. Thus GADD153 acts as a negative modulator of C/EBP-like proteins in certain terminally differentiated cells.
Synthetic peptide surrounding amino acid 124 of human GADD153
100 µg (0.2 mg/ml) Protein A purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.
-20 °C. The shelf life is 12 months.