Protein Name & Synonyms:
GOPC, CAL, FIG, GOPC1, PIST, dJ94G16.2, golgi associated PDZ and coiled-coil motif containing
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Human, Mouse, Rat
This gene encodes a Golgi protein with a PDZ domain. The PDZ domain is globular and proteins which contain them bind other proteins through short motifs near the C-termini. Mice which are deficient in the orthologous protein have globozoospermia and are infertile. Multiple transcript variants encoding different isoforms have been found for this gene.
WB 1:500 - 1:2000
IF 1:50 - 1:200
Recombinant fusion protein containing a sequence corresponding to amino acids 195-454 of human GOPC (NP_001017408.1).
Western blot analysis of extracts of various cell lines, using GOPC antibody (144-07513) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.
Immunofluorescence analysis of A549 cells using GOPC antibody (144-07513). Blue: DAPI for nuclear staining.