Rabbit anti-HBA2 Antibody

HBA2 Polyclonal Antibody

144-08427-50
+

Antigen Information

Accession Number:
  • P69905
Gene ID:
  • 3040
Gene Symbols:
  • HBA2
Protein Name & Synonyms:
HBA2, HBA-T2, HBH, hemoglobin subunit alpha
Target Species:
  • Human

Antibody Specifications

Applications:
WB,IHC
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugation/Tag:
Unconjugated
Host Species:
Rabbit
Purity:
Affinity purification
Species Reactivity:
Mouse,Rat
Clonality:
Polyclonal Antibodies
Isotype:
IgG

Background

The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:100

Format

Calculated MW
15kDa
Observed MW
13kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human HBA2 (NP_000508.1).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of mouse liver, using HBA2 antibody (144-08427) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Western blot - HBA2 antibody (144-08427)

Immunohistochemistry of paraffin-embedded mouse heart using HBA2 antibody (144-08427) at dilution of 1:100 (40x lens).
Immunohistochemistry - HBA2 antibody (144-08427)

Immunohistochemistry of paraffin-embedded mouse brain using HBA2 antibody (144-08427) at dilution of 1:100 (40x lens).
Immunohistochemistry - HBA2 antibody (144-08427)

No posts found

Write A Review
U.S. Non-U.S.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.