Protein Name & Synonyms:
HBA2, HBA-T2, HBH, hemoglobin subunit alpha
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
The human alpha globin gene cluster located on chromosome 16 spans about 30 kb and includes seven loci: 5'- zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 - alpha-1 - theta - 3'. The alpha-2 (HBA2) and alpha-1 (HBA1) coding sequences are identical. These genes differ slightly over the 5' untranslated regions and the introns, but they differ significantly over the 3' untranslated regions. Two alpha chains plus two beta chains constitute HbA, which in normal adult life comprises about 97% of the total hemoglobin; alpha chains combine with delta chains to constitute HbA-2, which with HbF (fetal hemoglobin) makes up the remaining 3% of adult hemoglobin. Alpha thalassemias result from deletions of each of the alpha genes as well as deletions of both HBA2 and HBA1; some nondeletion alpha thalassemias have also been reported.
WB 1:500 - 1:2000
IHC 1:50 - 1:100
Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human HBA2 (NP_000508.1).
Western blot analysis of extracts of mouse liver, using HBA2 antibody (144-08427) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.
Immunohistochemistry of paraffin-embedded mouse heart using HBA2 antibody (144-08427) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using HBA2 antibody (144-08427) at dilution of 1:100 (40x lens).