Protein Name & Synonyms:
HLA-DPB1, DPB1, HLA-DP, HLA-DP1B, HLA-DPB, major histocompatibility complex, class II, DP beta 1
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Human, Mouse, Rat
HLA-DPB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta chain (DPB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules.
WB 1:500 - 1:2000
IHC 1:50 - 1:200
Recombinant fusion protein containing a sequence corresponding to amino acids 30-225 of human HLA-DPB1 (NP_002112.3).
Western blot analysis of extracts of various cell lines, using HLA-DPB1 antibody (144-01412) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human lymphonodus using HLA-DPB1 antibody (144-01412) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using HLA-DPB1 antibody (144-01412) at dilution of 1:100 (40x lens).