Rabbit anti-HLA-DPB1 Antibody

HLA-DPB1 Polyclonal Antibody

$200.00  
144-01412-50
+

Antigen Information

Accession Number:
  • P04440
Gene ID:
  • 3115
Gene Symbols:
  • HLA-DPB1
Protein Name & Synonyms:
HLA-DPB1, DPB1, HLA-DP, HLA-DP1B, HLA-DPB, major histocompatibility complex, class II, DP beta 1
Target Species:
  • Human

Antibody Specifications

Applications:
WB, IHC
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Conjugation/Tag:
Unconjugated
Host Species:
Rabbit
Purity:
Affinity purification
Species Reactivity:
Human, Mouse, Rat
Clonality:
Polyclonal
Isotype:
IgG

Background

HLA-DPB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta chain (DPB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules.

Dilutions

WB 1:500 - 1:2000
IHC 1:50 - 1:200

Format

Calculated MW
29kDa
Observed MW
29kDa

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 30-225 of human HLA-DPB1 (NP_002112.3).

Modification

Unmodified

Storage

-20°C

Images

Western blot analysis of extracts of various cell lines, using HLA-DPB1 antibody (144-01412) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Western blot - HLA-DPB1 Polyclonal Antibody (144-01412)

Immunohistochemistry of paraffin-embedded human lymphonodus using HLA-DPB1 antibody (144-01412) at dilution of 1:100 (40x lens).
Immunohistochemistry - HLA-DPB1 Polyclonal Antibody (144-01412)

Immunohistochemistry of paraffin-embedded human stomach using HLA-DPB1 antibody (144-01412) at dilution of 1:100 (40x lens).
Immunohistochemistry - HLA-DPB1 Polyclonal Antibody (144-01412)

No posts found

Write A Review

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.