Rabbit Anti-Human 4EBP1 (C-term)

Rabbit Anti-Human 4EBP1 (C-term)

DS-PB-00023
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Antigen Information

Protein Name & Synonyms:
4EBP1
Target Species:
  • Human

Antibody Specifications

Applications:
Western Blotting
Concentration:
IgG concentration 1.0mg/ml
Preparation:
Antisera to human 4EBP1 (CT) were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Species Reactivity:
Human, Mouse

This product detects a band of approximately 20kDa in mouse 3T3 cell lysates. (predicted Mwt. 13kDa).

Specificity

This product recognizes an epitope within the C-terminal region (CT) of eukaryotic translation initiation factor 4E binding protein 1 (4EBP1), a rapamycin-sensitive downstream target of TOR (Target of rapamycin), and regulatory factor of eukaryotic mRNA translation.

The association between 4EPB1 and eukaryotic translation initiation factor 4E (eIF-4E) inhibits the binding of eIF-4E with p220, thereby preventing the formation of a functional eIF-4E complex, essential for mRNA translation. Phosphorylation of 4EBP1 following stimulation by mitogens such as insulin, results in the release of eIF-4E from 4EBP1, thereby enabling the translation complex to assemble.

This product has been reported as suitable for use in IHC on mouse 3T3 cell cytospins.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified

Preparation

Antisera to human 4EBP1 (CT) were raised by repeated immunisation of rabbits with highly purified antigen.

Purified IgG prepared by affinity chromatography.

Immunogen:
A 14 amino acid peptide located near human 4EBP1 carboxy-terminus.
Preservative:
0.02% Sodium Azide (NaN3)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.