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770-729-2992
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Product Specifications
Size:
0.1 mg
Protein Information
Protein Name & Synonyms:
4EBP1
Target Species:
- Human
Antibody Specifications
Applications:
Western Blotting
Concentration (lot specific):
IgG concentration 1.0mg/ml
Preparation:
Antisera to human 4EBP1 (CT) were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Species Reactivity:
Human, Mouse
This product detects a band of approximately 20kDa in mouse 3T3 cell lysates. (predicted Mwt. 13kDa).
Specificity
This product recognizes an epitope within the C-terminal region (CT) of eukaryotic translation initiation factor 4E binding protein 1 (4EBP1), a rapamycin-sensitive downstream target of TOR (Target of rapamycin), and regulatory factor of eukaryotic mRNA translation.
The association between 4EPB1 and eukaryotic translation initiation factor 4E (eIF-4E) inhibits the binding of eIF-4E with p220, thereby preventing the formation of a functional eIF-4E complex, essential for mRNA translation. Phosphorylation of 4EBP1 following stimulation by mitogens such as insulin, results in the release of eIF-4E from 4EBP1, thereby enabling the translation complex to assemble.
This product has been reported as suitable for use in IHC on mouse 3T3 cell cytospins.
The association between 4EPB1 and eukaryotic translation initiation factor 4E (eIF-4E) inhibits the binding of eIF-4E with p220, thereby preventing the formation of a functional eIF-4E complex, essential for mRNA translation. Phosphorylation of 4EBP1 following stimulation by mitogens such as insulin, results in the release of eIF-4E from 4EBP1, thereby enabling the translation complex to assemble.
This product has been reported as suitable for use in IHC on mouse 3T3 cell cytospins.
Storage/Stability
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Format
Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified
Preparation
Antisera to human 4EBP1 (CT) were raised by repeated immunisation of rabbits with highly purified antigen.
Purified IgG prepared by affinity chromatography.
Immunogen:
A 14 amino acid peptide located near human 4EBP1 carboxy-terminus.
Preservative:
0.02% Sodium Azide (NaN3)
Expiration:
12 months from the date of shipment when stored properly.
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
