Rabbit Anti-Human 5-Lipoxygenase (pSer523)

Rabbit Anti-Human 5-Lipoxygenase (pSer523)

$582.00  
DS-PB-02383
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Antigen Information

Protein Name & Synonyms:
5-Lipoxygenase (pSer523)
Target Species:
  • Human

Antibody Specifications

Applications:
Western Blotting
Preparation:
Antisera to phosphorylated 5-lipoxygenase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Species Reactivity:
Human, Rat

This product detects a doublet of approximately 80kDa in rat cortex lysate.

Specificity

This product specifically recognizes human 5-lipoxygenase (5-LO, also known as arachidonate 5-lipoxygenase) when phosphorylated at Ser523. 5-LO is an enzyme that catalyses the synthesis of leukotrienes (LT❏'s) from arachidonic acid (a polyunsaturated fatty acid), by the insertion of an oxygen into the arachidonic backbone. 5-LO requires calcium, ATP and the 5-LO-activating protein as cofactors for full activity. Leukotrienes are synthesised and secreted by leukocytes in response to immunologic or inflammatory stimuli, such as in asthma and allergic reactions. The biological effects of LT❏'s include leukocyte chemotaxis, pulmonary airway constriction, mucous hypersecretion, vasoconstriction and increased vascular permeability. I recent years 5-LO has also been implicated in the regulation of cell proliferation and apoptosis and has been found in a broad variety of cancer cells.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
10mM HEPES pH7.5, 150mM NaCl
Format Type:
Purified

Preparation

Antisera to phosphorylated 5-lipoxygenase were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.

Immunogen:
Synthetic phosphopeptide corresponding to an amino acid sequence within human 5-lipoxygenase which includes phosphorylated Ser523.
Preservative:
0.09%Sodium Azide
0.01%Bovine Serum Albumin
50%Glycerol

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.