ELISA (recommended work dilution= 1: 5,000-10,000), Western Blotting (recommended work dilution= 1:1000-2000)
Immunogen was synthetic peptide derived from Human Adipsin. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography.
A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial.
Adipsin is also called as complement factor D, or C3 convertase activator or properdin factor D, because they are identical molecule, mature protein aa 26-253, ~22kD. It is belonged to a serine protease that is secreted by adipocytes. Adipocytes synthesize and store energy in periods of nutritional abundance and mobilize lipids during starvation and other times of need. The switch from energy storage to expenditure is finely regulated by a variety of hormones. In order to accomplish these complex tasks energy balance, adipocytes express many genes, including adipsin, involved in lipid metabolism and glucose homeostasis. Many of these genes are finely regulated during adipocyte differentiation and maturation. Several adipocyte-derived proteins act in an autocrine or paracrine fashion to control its own and other cell's cellular physiology.
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
The rabbit anti-human Adipsin antibody detects targets derived from human recombinant and cellular proteins at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
- White R.T., Damm D., and et.al. Human adipsin is identical to complement factor D and is expressed at high levels in adipose tissue. J. Biol. Chem. 267:9210-9213(1992)
- Davis A.E. III, Active site amino acid sequence of human factor D. Proc. Natl. Acad. Sci. U.S.A. 77:4938-4942(1980)
12 months from the date of shipment when stored properly.