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770-729-2992
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Protein Information
Target Species:
- Human
Antibody Specifications
Applications:
ELISA (recommended work dilution= 1: 5,000), Western Blotting (recommended work dilution= 1: 1,000)
Conjugation/Tag:
Biotin
Host Species:
Rabbit
Preparation:
Immunogen was peptide near at C-terminus derived from human Amylin. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction of immunized serum was purified by Protein G/A affinity chromatography.
Reconstitution:
A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial.
Species Reactivity:
Human Amylin polyclonal antibody detects protein derived from human cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.
Clonality:
Polyclonal
Isotype:
IgG
Description
Adrenomedullin (AM), alpha- and beta-calcitonin gene-related peptide (CGRP), calcitonin (CT), and amylin are homologous polypeptides with overlapping biological actions such as vasodilatation and inhibition of bone resorption. Amylin (Islet/insulinoma amyloid polypeptide or IAPP) is a 37- amino acid monomeric polypeptide isolated from pancreatic amyloid. This protein, with a calculated molecular mass of 4 kDa, is a major component of amyloid-rich pancreatic extracts of three type 2 diabetic patients. IAPP has cysteine residues in positions 2 and 7, a feature found in all known calcitonin generelated peptides. IAPP shows 46% amino acid sequence homology with CGRP II. Since IAPP has been demonstrated immunochemically in normal beta cells of several mammals, it probably has an important role in respect to pancreatic islet function. The gene which encodes amylin maps to human chromosome 12p12.3-p12.1.
Storage / Stability
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
Specificity
Human Amylin polyclonal antibody detects protein derived from human cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.
References
- Dacquin, R., Davey, R.A., Laplace, C., Levasseur, R., Morris, H.A., Goldring, S.R., Gebre-Medhin, S., Galson, D.L., Zajac, J.D., Karsenty, G. Amylin inhibits bone resorption while the calcitonin receptor controls bone formation in vivo. J. Cell Biol. (2004)
- Janson, J., Soeller, W.C., Roche, P.C., Nelson, R.T., Torchia, A.J., Kreutter, D.K., Butler, P.C. Spontaneous diabetes mellitus in transgenic mice expressing human islet amyloid polypeptide. Proc. Natl. Acad. Sci. U.S.A. (1996)
Expiration:
12 months from the date of shipment when stored properly.
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
