ELISA (recommended work dilution= 1:2,000-5,000), Western Blotting( recommended work dilution= 2-5 µg/ml)
This antibody was produced from a rabbit immunized with a peptide derived from the C-terminal region of Human Apelin. The IgG fraction of immunized serum was purified by Protein A/G affinity chromatography.
A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial.
Apelin (APEL), an endogenous ligand for APJ, is an alternate coreceptor with CD4 for HIV-1 infection. This secreted protein inhibits HIV-1 entry into cells that coexpress APJ and CD4. By proteolytic processing of the peptide precursor, several different active peptides may be produced. Apelin-36, one such inotropic peptide, is being investigated as a potential plasma marker of cardiopulmonary disease. Apelin is highly expressed in brain, mainly in the thalamus, frontal cortex, hypothalamus and midbrain. Apelin is also secreted by the mammary gland into the colostrum and milk. Oral intake of Apelin (in milk and colostrum) may be important in the modulation of the immune responses in neonates and newborns. Apelin has also been found to be a potent stimulator of cardiac contractility and may function in the regulation of the cardiovascular system.
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
The rabbit anti-human Apelin antibody detects the human recombinant protein and the cellular protein from 3T3-L1 cell lysates at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
- Tatemoto, K., et al. 1998. Isolation and characterization of a novel endogenous peptide ligand for the human APJ receptor. Biochem. Biophys. Res. Commun. 251: 471-476.
- Habata, Y., et al. 1999. Apelin, the natural ligand of the orphan receptor APJ, is abundantly secreted in the colostrum. Biochim. Biophys. Acta 1452: 25-35.
12 months from the date of shipment when stored properly.