Rabbit Anti-Human Apelin ligand (N-terminus)

Rabbit Anti-Human Apelin ligand (N-terminus) Antibody

$52.00  
RB-08-0004-20
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Protein Information

Target Species:
  • Human

Antibody Specifications

Applications:
(under testing)Direct ELISA: antibody detection limit dilution will be 1:10,000-20,000. Western Blot: This antibody will be used at 1 - 2 µg/mL with the appropriate secondary reagents to detect human Apelin. Immunohistochemistry: under testing
Immunogen Sequence:
CLSEAGLKGPPSAH
Preparation:
Immunogen was peptide near at N-terminus derived from human Apelin. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction of immunized serum was purified by Protein G/A affinity chromatography.
Reconstitution:
Lyophilized rabbit anti-human Apelin antibody is stable at 4 °C and below -18 °C. Supplied as 200 µg of purified antibody. Reconstitute with sterile PBS. If 1 ml of PBS is used, the antibody concentration will be 200 µg/ml. Please avoid freeze-thaw cycles.
Species Reactivity:
Human Apelin polyclonal antibody detects protein derived from human cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.
Clonality:
Polyclonal

Description

Apelin (APEL), an endogenous ligand for APJ, is an alternate coreceptor with CD4 for HIV-1 infection. This secreted protein inhibits HIV-1 entry into cells that coexpress APJ and CD4. By proteolytic processing of the peptide precursor, several different active peptides may be produced. Apelin-36, one such inotropic peptide, is being investigated as a potential plasma marker of cardiopulmonary disease. Apelin is highly expressed in brain, mainly in the thalamus, frontal cortex, hypothalamus and midbrain. Apelin is also secreted by the mammary gland into the colostrum and milk. Oral intake of Apelin (in milk and colostrum) may be important in the modulation of the immune responses in neonates and newborns. Apelin has also been found to be a potent stimulator of cardiac contractility and may function in the regulation of the cardiovascular system.

Specificity

Human Apelin polyclonal antibody detects protein derived from human cell lysate at various concentrations. Cross reactivity with mouse and rat is not tested.

Storage

Lyophilized samples are stable for a year at least from date of receipt when stored at -20° C to -70° C. Upon reconstitution, the antibody can be stored at 2° - 8° C for at least a month without detectable loss of activity. Minimize freezing and thawing.

References

  • Tatemoto, K., et al. 1998. Isolation and characterization of a novel endogenous peptide ligand for the human APJ receptor. Biochem. Biophys. Res. Commun. 251: 471-476.
  • Habata, Y., et al. 1999. Apelin, the natural ligand of the orphan receptor APJ, is abundantly secreted in the colostrum. Biochim. Biophys. Acta 1452: 25-35.
Expiration:
12 months from the date of shipment when stored properly.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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