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Antigen Information
Protein Name & Synonyms:
ASK1 (pSer83)
Target Species:
- Human
Antibody Specifications
Applications:
ELISA (dilution: 1/5,000, 1/1,000), Western Blotting
Concentration:
IgG concentration 2.0mg/ml
Preparation:
Antisera to ASK1 (pSER83) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
Species Reactivity:
Human
This product detects a band of approximately 155kDa in SW1353 cell lysates.
Specificity
This product detects Apoptosis Signal-regulating Kinase 1 (ASK1) phosphorylated at serine83. ASK1 is a serine/threonine kinase also known as MAP kinase kinase kinase (MAPKKK), and plays an important role in the apoptotic response to various stimuli including oxidative stress, cytokines (e.g. TNF) and endoplasmic reticulum stress. Akt in conjunction to Hsp90 phosphorylates ASK1 at Ser83 to inhibit ASK1 induced apoptosis.
Storage/Stability
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Format
Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified
Preparation
Antisera to ASK1 (pSER83) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
Immunogen:
KLH conjugated peptide corresponding to amino acids 76-87 of human ASK-1 protein.
Preservative:
| 0.01% | Sodium Azide (NaN3) |
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
