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Product Specifications
Size:
50 µg
Protein Information
Protein Name & Synonyms:
BCA-1 / CXCL13
Target Species:
- Human
Antibody Specifications
Applications:
ELISA (dilution: 0.1, 0.2ug/ml), Western Blotting
Concentration (lot specific):
0.1 mg/ml after reconstitution
Preparation:
Antisera to human CXCL13 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Reconstitution:
Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. N.B. For long term storage the addition of 0.09% sodium azide is recommended.
Species Reactivity:
Human
Specificity
This product is specific for human BCA-1 (B cell attractant chemokine), a 10.1kDa CXC cytokine designated CXCL13, which acts as a potent chemoattractant for B lymphocytes, and is expressed at high levels in the liver and to a lesser extent by lymph nodes, spleen, stomach, appendix and salivary glands.
Studies have revealed that BCA-1 functions as a ligand for Burkitt❏'s lymphoma receptor 1 (BLR-1), a chemokine receptor designated CXCR5, expressed by all mature B lymphocytes.
Studies have revealed that BCA-1 functions as a ligand for Burkitt❏'s lymphoma receptor 1 (BLR-1), a chemokine receptor designated CXCR5, expressed by all mature B lymphocytes.
Storage/Stability
Prior to reconstitution store at +4 °C.
After reconstitution store at -20 °C.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Format
Supplied as:
Purified IgG conjugated to Biotin - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.5ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution.
N.B. For long term storage the addition of 0.09% sodium azide is recommended.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution.
N.B. For long term storage the addition of 0.09% sodium azide is recommended.
Preparation
Antisera to human CXCL13 were raised by repeated immunisations of rabbits with highly purified antigen.
Purified IgG was prepared from whole serum by affinity chromatography.
Immunogen:
Recombinant human CXCL13.
Preservative:
None present
Expiration:
12 months from the date of shipment when stored properly.
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
