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Antigen Information
Protein Name & Synonyms:
Beclin-1
Target Species:
- Human
Antibody Specifications
Applications:
Flow Cytometry, Immunohistology - Paraffin, Western Blotting
Concentration:
IgG concentration 1.0mg/ml
Preparation:
Antisera to human Beclin-1 (NT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Species Reactivity:
Human, Mouse and rat
This product detects a band of approximately 52kDa in human 293 cell lysates.
Specificity
This product recognizes an epitope within the N-terminal region of Beclin-1, a 52kDa coiled-coil protein which shares structural homology with the yeast autophagy gene apg6/vps30.
The ability of cells to undergo bulk degradation of endogenous proteins through an autophagosomic-lysosomal pathway, termed autophagy or Type II cell death, is important in the control of cell growth and development. Studies have identified Beclin-1 as a Bcl-2 interacting mammalian gene which not only mediates autophagy, but also functions as a tumour suppressor, being expressed at reduced levels in human breast carcinoma, and may also play a role in defense against viral infection. An increasing number of studies have revealed an interaction between proteins which induce autophagy and those involved in apoptosis and in some instances apoptosis may be dependant upon or delayed by autophagy.
The ability of cells to undergo bulk degradation of endogenous proteins through an autophagosomic-lysosomal pathway, termed autophagy or Type II cell death, is important in the control of cell growth and development. Studies have identified Beclin-1 as a Bcl-2 interacting mammalian gene which not only mediates autophagy, but also functions as a tumour suppressor, being expressed at reduced levels in human breast carcinoma, and may also play a role in defense against viral infection. An increasing number of studies have revealed an interaction between proteins which induce autophagy and those involved in apoptosis and in some instances apoptosis may be dependant upon or delayed by autophagy.
Storage/Stability
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Format
Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified
Preparation
Antisera to human Beclin-1 (NT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Immunogen:
A 17 amino acid peptide located near human Beclin-1 amino-terminus.
Preservative:
| 0.02% | Sodium Azide (NaN3) |
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
