Antigen Catalog Number:
ELISA (recommended work dilution= 1: 80,000) , Western Blotting (recommended work dilution= 1: 1,000-5,000) , Immunodetection Analysis: Representative blot from a previous lot. Lane 1, recombinant protein BTC-beta. The membrane blot was probed with anti BTC-beta primary antibody (0.2 µg/ml). Proteins were visualized using a Donkey anti-rabbit secondary antibody conjugated to IRDye 800CW detection system. Arrows indicate recombinant protein BTC-beta from E.coli cell (20kDa).
The immunogen was a recombinant protein. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum followed by Protein A/G affinity chromatography.
Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile 1x PBS to a final concentration of 1 mg/ml.
Betacellulin (BTC) is a member of the epidermal growth factor (EGF) family which binds and activates the EGF receptor and erbB-4, synthesized primarily as a transmembrane precursor.
This antibody binds to its immunogen, and did not show any cross reactivity with unrelated antigens in ELISA. Cross reactivity with mouse and rat BTCbeta has not been tested yet.
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -81°C. After reconstitution, it can be aliquoted and stored
- MarÃa Victoria GÃ³mez-Gaviro, et al. Betacellulin promotes cell proliferation in the neural stem cellniche and stimulates neurogenesis. Proc Natl Acad Sci U S A. 2012 January 24; 109(4): 13171322.
- Bela Anand-Apte, et al. Betacellulin Induces Increased Retinal Vascular Permeability in Mice. PLoSOne. 2010; 5(10): e13444.