Rabbit Anti-Human BIK (N-term)

Rabbit Anti-Human BIK (N-term)
CODE:  DS-PB-00270

Antigen Information

Protein Name & Synonyms:
BIK
Target Species:
  • Human

Antibody Specifications

Applications:
Immunohistology - Frozen, Western Blotting
Concentration:
IgG concentration 1.0mg/ml
Preparation:
Antisera to Bik were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Species Reactivity:
Human, Expected cross reactivity from sequence similarity: mouse and rat

This product detects a band of approximately 18kDa in Jurkat cell lysates.

Specificity

This product detects an epitope within the N-terminal (NT) region of Bcl-2-interacting killer (Bik) also known as natural born killer (Nbk). Bik is a Bcl-2 homology domain 3 (BH3)-only protein of 18kD which requires the BAX gene for activation. Bik forms heterodimers with various antiapoptotic proteins such as Bcl-2 and Bcl-XL, inhibiting their antiapoptotic function. This can result in the release of Ca2+ and cytochrome C into the cytosol, leading to apoptosis.

Loss of Bik is a common feature of clear-cell renal cell carcinoma (RCC), and is thought to act as a renal tissue-specific tumour suppressor gene.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified

Preparation

Antisera to Bik were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.

Immunogen:
15 amino acid peptide from near the amino terminus of human Bik.
Preservative:
0.02%Sodium Azide