ELISA (recommended work dilution= 1:2000-8000), Enzyme Immunoassay (EIA)(recommended work dilution= Sensitivity (772 µg/ml))
Immunogen was synthetic peptide derived from Human C-peptide. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography.
A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial.
C-peptide is a 31-amino acid single-chain peptide that is made when proinsulin is split into insulin and C-peptide. They split before proinsulin is released from endocytic vesicles within the pancreas one C-peptide for each insulin molecule. C-peptide is the middle segment of proinsulin that is between the N-terminal B-chain (30amino acids) and the C-terminal A-chain (21 amino acids).Unlike insulin, Cpeptide has no known physiological function. Due to the fact that C-peptide has a 2-to-5-time longer half-life than insulin, there are higher concentrations of C-peptide than insulin in the peripheral circulation.
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
The rabbit anti-human C-peptide antibody detected specifically target derived from human samples, such as serum and cell lysates at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
C-Peptide EIA Kit for Serum, Plasma, Culture Supernantant and Cell Lysates,Cat# EIA-CPE-1
- Kamiya H, Zhang W, Sima AA (2004). "C-peptide prevents nociceptive sensory neuropathy in type 1 diabetes". Ann. Neurol.56 (6): 82735.
- Martina V, Maestroni A, Maestroni S, Zerbini G. Role of C-peptide in the pathogenesis of diabetic nephropathy. G Ital Nefrol. 2010 May-Jun;27(3):240-8.