ELISA (recommended work dilution= 1:2000-8000), Enzyme Immunoassay (EIA)(recommended work dilution= Sensitivity (772 pg/ml))
Immunogen was synthetic peptide derived from Human C-peptide. This antibody was produced from a rabbit immunized with the immunogen. The IgG fraction was purified from rabbit serum by ammonium sulphate precipitation followed by Protein A/G affinity chromatography.
Supplied as lyophilized and purified antibody originally containing PBS, without Preservative Stabilizers, such as Sodium Azide. The final concentration is indicated on the shipping vial. The antibody is stable for at least years from the data of receipt when stored at -200 C to -700 C. Reconstituted antibody (recommended with H20 1 mg/ml) can also be aliquoted and stored frozen at -200 C to -700 C in a manual defrost freezer for months without detectable loss activity. Upon reconstitution, the antibody can be stored at 40 C for 1 month. Please avoid freeze-thaw cycles.
C-peptide is a 31-amino acid single-chain peptide that is made when proinsulin is split into insulin and C-peptide. They split before proinsulin is released from endocytic vesicles within the pancreas one C-peptide for each insulin molecule. C-peptide is the middle segment of proinsulin that is between the N-terminal B-chain (30amino acids) and the C-terminal A-chain (21 amino acids).Unlike insulin, Cpeptide has no known physiological function. Due to the fact that C-peptide has a 2-to-5-time longer half-life than insulin, there are higher concentrations of C-peptide than insulin in the peripheral circulation.
The rabbit anti-human C-peptide antibody detected specifically target derived from human samples, such as serum and cell lysates at various concentrations. Cross reactivity with mouse and rat are expected from sequence similarity.
Store at -200 C, if not intended for use within a month, Keep it at 40 C and minimize freezing and thawing when use.
C-Peptide EIA Kit for Serum, Plasma, Culture Supernantant and Cell Lysates,Cat# EIA-CPE-1
- Kamiya H, Zhang W, Sima AA (2004). "C-peptide prevents nociceptive sensory neuropathy in type 1 diabetes". Ann. Neurol.56 (6): 82735.
- Martina V, Maestroni A, Maestroni S, Zerbini G. Role of C-peptide in the pathogenesis of diabetic nephropathy. G Ital Nefrol. 2010 May-Jun;27(3):240-8.