Rabbit Anti-Human CASP1 (C-term)

Rabbit Anti-Human CASP1 (C-term)

DS-PB-00372
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Antigen Information

Protein Name & Synonyms:
CASP1
Target Species:
  • Human

Antibody Specifications

Applications:
Immunohistology - Paraffin, Western Blotting
Concentration:
IgG concentration 1.0 mg/ml
Preparation:
Antisera to human Caspase-1 (CT) were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Species Reactivity:
Human
Immunohistology:
*This product does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining.

Specificity

This product recognizes an epitope within the C-terminal region (CT) of human Caspase-1, otherwise known as IL-1Beta converting enzyme. Caspase-1 is an intracellular cysteine protease, identified as a mammalian homologue to C. elegans cell death gene (ced-3).

Caspase-1 has been classified as an inflammatory, rather than apoptotic caspase, due to its essential role in the cleavage of the inactive precursors of the cytokines IL-1beta and IL-18, into their mature activated and secretable forms.

This regulation of pro-inflammatory cytokines by Caspase-1 has made inhibitors of Caspase-1 a possible target for use as therapeutic drugs for the treatment of inflammatory diseases (1,2).

This product detects a cleaved subunit band of approximately 21 kDa in human heart cell lysates (predicted precursor MWT 45.2kDa).

Storage/Stability

Store at +4 °C or at -20 °C if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified

Preparation

Antisera to human Caspase-1 (CT) were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.

Immunogen:
A 16 amino acid peptide located near human Caspase-1 carboxy terminus.
Preservative:
0.02%Sodium Azide

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.