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770-729-2992
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Product Specifications
Size:
0.1 mg
Protein Information
Protein Name & Synonyms:
CD253
Target Species:
- Human
Antibody Specifications
Applications:
ELISA (dilution: 0.1, 0.2ug/ml), Immunohistology - Paraffin, Western Blotting
Concentration (lot specific):
IgG concentration 1.0mg/ml after reconstitution.
Preparation:
Antisera to human CD253 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Reconstitution:
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended.
Species Reactivity:
Human
Specificity
This product is specific for human CD253, otherwise known as TRAIL or Apo2L, a 281 amino acid type II transmembrane glycoprotein and member of the tumour necrosis factor superfamily, which induces apoptosis in tumour and transformed cells, but not normal cells.
Activation of apoptosis by CD253 occurs through binding to the death-signaling receptors DR4 (TRAILR1) and DR5 (TRAILR2), leading to formation of the death-inducing signaling complex (DISC) and the triggering of a proteolytic caspase cascade. There are however decoy receptors known as DcR1 (TRAILR3) and DcR2 (TRAILR4) which competitively bind to CD253 and are unable to initiate apoptosis.
CD253 also plays a role in immune surveillance, acting as a mediator of monocyte, NK cell, dendritic cell and T cell anti-tumour and anti-viral cytotoxicity.
Activation of apoptosis by CD253 occurs through binding to the death-signaling receptors DR4 (TRAILR1) and DR5 (TRAILR2), leading to formation of the death-inducing signaling complex (DISC) and the triggering of a proteolytic caspase cascade. There are however decoy receptors known as DcR1 (TRAILR3) and DcR2 (TRAILR4) which competitively bind to CD253 and are unable to initiate apoptosis.
CD253 also plays a role in immune surveillance, acting as a mediator of monocyte, NK cell, dendritic cell and T cell anti-tumour and anti-viral cytotoxicity.
Storage/Stability
Prior to reconstitution store at +4 °C.
After reconstitution store at -20 °C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Format
Supplied as:
Purified IgG - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended.
Format Type:
Purified
Preparation
Antisera to human CD253 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Immunogen:
Recombinant human CD253
Preservative:
None present
Expiration:
12 months from the date of shipment when stored properly.
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
