Rabbit Anti-Human CD254 [+Biotin]

Rabbit Anti-Human CD254 [+Biotin] (50 ug)

DS-PB-00424
+

Antigen Information

Protein Name & Synonyms:
CD254
Target Species:
  • Human

Antibody Specifications

Applications:
ELISA (dilution: 0.1, 0.2ug/ml), Western Blotting
Concentration:
IgG concentration 50ug/ml
Preparation:
Antisera to human CD254 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Reconstitution:
Reconstitute with 1.0ml sterile PBS containing 0.1% Bovine Serum Albumin.. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Species Reactivity:
Human

Specificity

This product is specific for the soluble form of human CD254, otherwise known as sRANKL, a 20kDa glycoprotein and member of the Tumour Necrosis Factor (TNF) superfamily, generated by proteolytic processing of the full-length CD254 membrane protein.

CD254 is highly expressed by T lymphocytes and osteoblastic cells and plays a key role in the differentiation and activation of osteoclast precursors, acting as a ligand for both receptor activator of NF-KBeta (RANK) and the decoy receptor osteoprotegerin (OPG), which act as antagonists. Binding of CD254 to RANK is essential for the activation of osteoclastogenesis, stimulated by factors such as TNF-alpha, IL-1beta, IL-6 and IL-17, whereas binding to OPG neutralizes CD254, thereby inhibiting osteoclastogenesis.

Studies have shown that CD254 augments the dendritic cell (DC) stimulation of naive T cell proliferation and may be involved in the regulation of DC-T cell interactions. Studies have also revealed a link between CD254 and apoptosis, through activation of the antiapoptotic kinase AKT/PKB and the mTOR/S6K signalling pathway.

Storage/Stability

Prior to reconstitution store at +4 °C.
After reconstitution store at -20 °C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:
Purified IgG conjugated to Biotin - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 1.0ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.

Preparation

Antisera to human CD254 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.

Immunogen:
Recombinant human CD254
Preservative:
None present

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.