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770-729-2992
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Product Specifications
Size:
0.1 mg
Protein Information
Protein Name & Synonyms:
CD286
Target Species:
- Human
Antibody Specifications
Applications:
Immunohistology - Frozen, Western Blotting
Concentration (lot specific):
IgG concentration 1.0mg/ml
Preparation:
Antisera to human CD286 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Species Reactivity:
Human
This product detects a band of approximately 98kDa and 110kDa in human Jurkat cell lysates (predicted Mwt. 91.9kDa). Doublet band may be due to protein modification or the recognition of two different isoforms by the same gene.
Specificity
This product is specific for human CD286, also known as Toll-like receptor 6 (TLR6), a member of the evolutionarily conserved toll-like receptor family (TLRs) and close homologue of CD281 (TLR1), that is expressed by monocytes, CD11c+ immature dendritic cells and endothelial cells of skin microvessels.
TLRs play an important role in the recognition of invading microbial pathogens during innate immunity, acting through adaptor molecules such as MyD88 and TRAF6. CD286 acts via a cytokine signaling pathway to induce the activation of NF-kappaB, through the formation of dimers with CD282 (TLR2) which coordinate macrophage activation by Gram-positive bacteria and zymosan. Studies have also revealed the involvement of CD286/CD282 dimers in apoptotic responses, through recognition of mycoplasmal lipoproteins.
This product does not recognize CD281 (TLR1).
This product has been shown as suitable for use in IHC on Jurkat cell cytospins at 2ug/ml.
TLRs play an important role in the recognition of invading microbial pathogens during innate immunity, acting through adaptor molecules such as MyD88 and TRAF6. CD286 acts via a cytokine signaling pathway to induce the activation of NF-kappaB, through the formation of dimers with CD282 (TLR2) which coordinate macrophage activation by Gram-positive bacteria and zymosan. Studies have also revealed the involvement of CD286/CD282 dimers in apoptotic responses, through recognition of mycoplasmal lipoproteins.
This product does not recognize CD281 (TLR1).
This product has been shown as suitable for use in IHC on Jurkat cell cytospins at 2ug/ml.
Storage/Stability
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Format
Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified
Preparation
Antisera to human CD286 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Immunogen:
A synthetic peptide corresponding to a 13 amino acid sequence located near the center of human CD286.
Preservative:
0.02% Sodium Azide (NaN3)
Expiration:
12 months from the date of shipment when stored properly.
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
