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Antigen Information
Protein Name & Synonyms:
Collagen I/III
Target Species:
- Human
Antibody Specifications
Applications:
Immunohistology - Frozen, ELISA (dilution: , 1/20), Immunofluorescence
Concentration:
IgG concentration 0.1 mg/ml after reconstitution.
Preparation:
Antiserum to human collagen type I and III was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Reconstitution:
Reconstitute with 0.5 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Species Reactivity:
Human
Specificity
This product is specific for human collagen types I and III, showing minimum cross reactivity with the following:
This antibody has been cross adsorbed against human plasma proteins. Human plasma proteins do not interfere with the binding of This product to collagen.
| Human collagen type IV | >3% |
| Human collagen type V | >2% |
This antibody has been cross adsorbed against human plasma proteins. Human plasma proteins do not interfere with the binding of This product to collagen.
Storage/Stability
Prior to reconstitution store at +4 °C.
After reconstitution store at -20 °C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Format
Supplied as:
Purified IgG - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. RayBiotech recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Format Type:
Purified
Preparation
Antiserum to human collagen type I and III was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Immunogen:
Collagen types I and III from human placenta
Preservative:
Mannitol, dextran and salts.
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Usage
This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.
