Rabbit Anti-Human DAI

Rabbit Anti-Human DAI
CODE:  129-10225

Antigen Information

Protein Name & Synonyms:
DAI
Target Species:
  • Human

Antibody Specifications

Applications:
ELISA (dilution: 0.5, 1.0ug/ml), Immunohistology - Paraffin, Western Blotting
Concentration:
1.0mg/ml
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat

This product detects a band of approximately 46 kDa in mouse small intestine tissue lysate

Specificity

This product recognizes human DAI, also known as Z-DNA Binding Protein (ZBP1), a 46kDa DNA binding protein belonging to a family of proteins that contain a Za domain which specifically binds to the left-handed Z-DNA and Z-RNA.

Initial studies suggested that DAI may be involved in processes such host response to neoplasia, additional research has indicated that the cytosolic DAI can act like the toll-like receptor TLR9 by detecting cytosolic double-stranded (ds) DNA and trigger induction of type I interferon and other innate immune responses. It is thought that the binding of DAI to dsDNA enhances its association with innate immune response proteins such as the IRF3 transcription factor and the serine/threonine kinase TBK1 (also known as NAK).

DAI is highly expressed in lymphatic tissues such as the lymph node, leukocytes, tonsil, bone marrow and spleen. Expression at a lower level is also seem in thymus, lung and liver. It has been demonstrated that multiple isoforms of DAI exist.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified

Preparation

Antisera to human DAI were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.

Immunogen:
Synthetic peptide corresponding to an 18 amino acid sequence from near the carboxy terminus of human ZBP1.
Preservative:
0.02% Sodium Azide (NaN3)