Rabbit Anti-Human EGF

Rabbit Anti-Human EGF

DS-PB-00693
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Antigen Information

Protein Name & Synonyms:
EGF
Target Species:
  • Human

Antibody Specifications

Applications:
ELISA (dilution: 0.1, 0.2ug/ml), Functional Assays, Immunohistology - Paraffin, Western Blotting
Concentration:
IgG concentration 1.0mg/ml after reconstitution
Preparation:
Antisera to human EGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Reconstitution:
Reconstitute with 0.1ml distilled water.For long term storage the addition of 0.09% sodium azide is recommended.N.B. For functional studies do not add azide.
Species Reactivity:
Human

Specificity

This product is specific for human EGF (Epidermal Growth Factor), a potent stimulator and regulator of the proliferation of epidermal and epithelial cells, which is expressed by a variety of cells.

The effects of EGF are initiated through tyrosine kinase activity, following the binding of EGF to the extracellular domain of the 170kDa EGF receptor (EGFR), which also binds other EGF-like ligands with high affinity, including TGF-α, VGF (vaccinia virus growth factor) and Betacellulin, produced by certain tumour cells. Receptor activation through EGF binding triggers several signal transduction pathways, including the JAK/STAT and P13K/AKT pathways.

Storage/Stability

Prior to reconstitution store at +4 °C.
After reconstitution store at -20 °C.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:
Purified IgG - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.1ml distilled water.
For long term storage the addition of 0.09% sodium azide is recommended.
N.B. For functional studies do not add azide.
Format Type:
Purified

Preparation

Antisera to human EGF were raised by repeated immunisations of rabbits with highly purified antigen.
Purified IgG prepared by affinity chromatography.

Immunogen:
Recombinant human EGF
Preservative:
None present.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.