Protein Name & Synonyms:
ENA-78 / CXCL5
Applications:
ELISA (dilution: 0.1, 0.2ug/ml), Functional Assays, Immunohistology - Paraffin, Western Blotting
Concentration:
IgG concentration 1.0mg/ml
Preparation:
Antisera to human CXCL5 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Reconstitution:
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Species Reactivity:
Human
This product is specific for human CXCL5, otherwise known as ENA-78, (epithelial-derived neutrophil-activating protein 78), a CXC chemokine expressed by monocytes, mast cells, platelets, eosinophils and endothelial cells, which acts as a key modulator of the inflammatory response, but also exhibits angiogenic properties, including tumour progression.
CXCL5 signals through binding to CXC receptor 2 (CXCR2) and occurs naturally in three isoforms: ENA 5-78 (74 amino acids), ENA 9-78 (70 amino acids) and ENA 10-78 (69 amino acids).
Prior to reconstitution store at +4 °C.
After reconstitution store at -20 °C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Supplied as:
Purified IgG - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Antisera to human CXCL5 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Immunogen:
Recombinant human CXCL5.
Preservative:
None present