Rabbit Anti-Human ENA-78 / CXCL5

Rabbit Anti-Human ENA-78 / CXCL5
CODE:  DS-PB-02578

Antigen Information

Protein Name & Synonyms:
ENA-78 / CXCL5
Target Species:
  • Human

Antibody Specifications

Applications:
ELISA (dilution: 0.1, 0.2ug/ml), Functional Assays, Immunohistology - Paraffin, Western Blotting
Concentration:
IgG concentration 1.0mg/ml
Preparation:
Antisera to human CXCL5 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Reconstitution:
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Species Reactivity:
Human

Specificity

This product is specific for human CXCL5, otherwise known as ENA-78, (epithelial-derived neutrophil-activating protein 78), a CXC chemokine expressed by monocytes, mast cells, platelets, eosinophils and endothelial cells, which acts as a key modulator of the inflammatory response, but also exhibits angiogenic properties, including tumour progression.

CXCL5 signals through binding to CXC receptor 2 (CXCR2) and occurs naturally in three isoforms: ENA 5-78 (74 amino acids), ENA 9-78 (70 amino acids) and ENA 10-78 (69 amino acids).

Storage/Stability

Prior to reconstitution store at +4 °C.
After reconstitution store at -20 °C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:
Purified IgG - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
Format Type:
Purified

Preparation

Antisera to human CXCL5 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.

Immunogen:
Recombinant human CXCL5.
Preservative:
None present