Protein Name & Synonyms:
bFGF / FGF basic / FGF2
Applications:
ELISA (dilution: 0.1, 0.2 ug/ml), Functional Assays, Immunohistology - Paraffin, Western Blotting
Concentration (lot specific):
IgG concentration 1.0 mg/ml after reconstitution
Preparation:
Antisera to human FGF basic were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Reconstitution:
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB. For functional studies do not add azide.
Species Reactivity:
Human
This product is specific for human FGF basic, otherwise known as FGF 2 (fibroblast growth factor 2), a member of the heparin-binding growth factor family which exists in both cytosolic and nuclear isoforms, ranging in size from 18-24kDa, expressed by the majority of cells and tissues.
FGF 2 is a multi-functional growth factor identified as a potent inducer of angiogenesis, an important factor in wound healing, tumour vascularisation and cardiovascular disease and is pivotal for the development and maintenance of vascular integrity during embryogenesis.
Prior to reconstitution store at +4 °C.
After reconstitution store at -20 °C.
Storage in frost-free freezers is not recommended. This product should be storde undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.
Supplied as:
Purified IgG - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB. For functional studies do not add azide.
Antisera to human FGF basic were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Immunogen:
Recombinant human FGF basic
Preservative:
None present.
Expiration:
12 months from the date of shipment when stored properly.