Rabbit Anti-Human G-CSF / CSF3

Rabbit Anti-Human G-CSF / CSF3

DS-PB-00855
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Antigen Information

Protein Name & Synonyms:
G-CSF / CSF3
Target Species:
  • Human

Antibody Specifications

Applications:
ELISA (dilution: 0.1, 0.2ug/ml), Functional Assays, Immunohistology - Paraffin, Western Blotting
Concentration:
IgG concentration 1.0mg/ml after reconstitution
Preparation:
Antisera to human G-CSF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Reconstitution:
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB. For functional studies do not add azide.
Species Reactivity:
Human

Specificity

This product is specific for human G-CSF (Granulocyte colony-stimulating factor), a 19.6kDa hematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which stimulates the development of bone marrow progenitor cells and in particular those committed to neutrophil lineage.

The ability of G-CSF to stimulate the proliferation/differentiation and function of neutrophil granulocyte progenitor cells and survival of mature neutrophils, has resulted in G-CSF becoming an important factor in patient treatment following allogeneic (bone marrow/stem cell) transplantation and chemotherapy.

To yield one-half maximal inhibition [ND50] of the biological activity of hG-CSF (0.5 ng/ml), a concentration of 0.04-0.06 ug/ml of this antibody is required.

Storage/Stability

Prior to reconstitution store at +4 °C.
After reconstitution store at -20 °C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 12 months after receipt.

Format

Supplied as:
Purified IgG - lyophilized
Formulation:
Phosphate buffered saline
Reconstitution:
Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB. For functional studies do not add azide.
Format Type:
Purified

Preparation

Antisera to human G-CSF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.

Immunogen:
Recombinant human G-CSF
Preservative:
None present

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.