Rabbit Anti-Human GAPDH (C-term)

Rabbit Anti-Human GAPDH (C-term)

$420.00
DS-PB-00847
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Antigen Information

Protein Name & Synonyms:
GAPDH
Target Species:
  • Human

Antibody Specifications

Applications:
Western Blotting
Concentration:
IgG concentration 1.0mg/ml
Preparation:
Antisera to human GAPDH (CT) were raised by repeated immunisation of rabbits with highly purified antigen.Purified IgG prepared by affinity chromatography.
Species Reactivity:
Human, Mouse and rat, Rat

This product detects a band of approximately 37kDa in HeLa cell lysates.

Specificity

This product recognizes an epitope within the C-terminal region (CT) of Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a glycolytic enzyme which plays a key role in energy production, and has also been implicated in numerous cellular processes.

GAPDH is a homotetramer molecule consisting of four 36kDa subunits, constitutively expressed in most cells and tissues, and is responsible for the generation of energy during carbohydrate metabolism, catalysing the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate.

The role of GAPDH as a multifunctional protein has emerged from many studies, confirming its involvement in critical nuclear pathways, apoptosis, membrane transport and fusion, DNA replication and repair and phosphotransferase activity. Furthermore, the ability of GAPDH to bind with high affinity to ß-amyloid precursor protein (Alzheimer's disease) and selectively with CAG mutated proteins, including huntingtin (Huntingdon❏'s disease) and the androgen receptor (spinobulbar muscular atrophy), has focused many studies towards the role of GAPDH in the pathogenesis of neurodegenerative diseases, see De Santo, C. et al. for details.

Storage/Stability

Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.

Format

Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Format Type:
Purified

Preparation

Antisera to human GAPDH (CT) were raised by repeated immunisation of rabbits with highly purified antigen.

Purified IgG prepared by affinity chromatography.

Immunogen:
A 16 amino acid peptide located near human GAPDH carboxy-terminus.
Preservative:
0.02% Sodium Azide (NaN3)

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.