ELISA (recommended work dilution= 1:64,000)
The immunogen was a synthetic peptide (Gastrin-I). This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified from rabbit serum by Protein G affinity chromatography.
A separate vial of dilution buffer is provided for reconstitution. The antibody is supplied lyophilized, originally containing PBS, without preservative stabilizers (e.g. sodium azide). The final amount is indicated on the shipping vial.
Gastrin is a peptide hormone that stimulates secretion of gastric acid (HCl) by the parietal cells of the stomach and aids in gastric motility. It is released by G cells in the antrum of the stomach, duodenum, and the pancreas. It binds to cholecystokinin B receptors to stimulate the release of histamines in enterochromaffin-like cells, and it induces the insertion of K+ /H+ ATPase pumps into the apical membrane of parietal cells (which in turn increases H+ release). Its release is stimulated by peptides in the lumen of the stomach. Gastrin is synthesized as a 101 residue prepropeptide on the rough endoplasmic reticulum, and then post-translationally modified by cleavage and alpha-amidation to result in the active forms: gastrin-34 ("big gastrin"); gastrin-17 ("little gastrin"); gastrin-14 ("minigastrin"). Gastrin-I is gastrin-17 ("little gastrin").
The antibody is stable for at least 1 year from the date of receipt when stored at -20°C to -70°C. Reconstituted antibody can also be aliquotted and stored at 4°C for 1 month or at -20°C to -70°C in a manual defrost freezer for many months without detectable loss activity. Please avoid freeze-thaw cycles.
This antibody was selected for its ability to specifically detect Gastrin-I from humans. This antibody can cross-react with Mouse and Rat Gastrin-I.
- Gregory, H. et al. (1964). "The antral Hormone Gastrin: Structure of Gastrin". Nature 204 (4962): 931.
- Wiborg, O. et al. (1984). "Structure of a human gastrin gene". Proc. Natl. Acad. Sci. U.S.A. 81 (4): 10679.
- Lund, T., et al., (1986). "The genes for human gastrin and cholecystokinin are located on different chromosomes". Hum. Genet. 73 (1): 7780.