ELISA (recommended work dilution= 1:64,000)
The immunogen was a synthetic peptide (Gastrin-I). This antibody was produced from a rabbit immunized with this peptide conjugated with KLH. The IgG fraction was purified from rabbit serum by Protein G affinity chromatography.
Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with 1 x PBS to a final concentration of 1 mg/ml.
Gastrin is a peptide hormone that stimulates secretion of gastric acid (HCl) by the parietal cells of the stomach and aids in gastric motility. It is released by G cells in the antrum of the stomach, duodenum, and the pancreas. It binds to cholecystokinin B receptors to stimulate the release of histamines in enterochromaffin-like cells, and it induces the insertion of K+ /H+ ATPase pumps into the apical membrane of parietal cells (which in turn increases H+ release). Its release is stimulated by peptides in the lumen of the stomach. Gastrin is synthesized as a 101 residue prepropeptide on the rough endoplasmic reticulum, and then post-translationally modified by cleavage and alpha-amidation to result in the active forms: gastrin-34 ("big gastrin"); gastrin-17 ("little gastrin"); gastrin-14 ("minigastrin"). Gastrin-I is gastrin-17 ("little gastrin").
This antibody was selected for its ability to specifically detect Gastrin-I from humans. This antibody can cross-react with Mouse and Rat Gastrin-I.
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for at least 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 1 month at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.
- Gregory, H. et al. (1964). "The antral Hormone Gastrin: Structure of Gastrin". Nature 204 (4962): 931.
- Wiborg, O. et al. (1984). "Structure of a human gastrin gene". Proc. Natl. Acad. Sci. U.S.A. 81 (4): 10679.
- Lund, T., et al., (1986). "The genes for human gastrin and cholecystokinin are located on different chromosomes". Hum. Genet. 73 (1): 7780.